The aim of this project was to examine the effect of microneedle rollers on the percutaneous\npenetration of tiagabine hydrochloride and carbamazepine across porcine skin in vitro. Liquid\nchromatography-mass spectrometric analysis was carried out using an Agilent 1200 Series HPLC\nsystem coupled to an Agilent G1969A TOF-MS system. Transdermal flux values of the drugs\nwere determined from the steady-state portion of the cumulative amount versus time curves.\nFollowing twelve hours of microneedle roller application, there was a 6.74-fold increase in the\npercutaneous penetration of tiagabine hydrochloride (86.42 �± 25.66 �¼g/cm2/h) compared to passive\ndelivery (12.83 �± 6.30 �¼g/cm2/h). For carbamazepine in 20% ethanol, passive transdermal flux of\n7.85 �± 0.60 �¼g/cm2/h was observed compared to 10.85 �± 0.11 �¼g/cm2/h after microneedle treatment.\nCarbamazepine reconstituted in 30% ethanol resulted in only a 1.19-fold increase in drug permeation\nacross porcine skin (36.73 �± 1.83 �¼g/cm2/h versus 30.74 �± 1.32 �¼g/cm2/h). Differences in flux values\nof untreated and microneedle-treated porcine skin using solid microneedles for the transdermal\ndelivery of tiagabine were statistically significant. Although there were 1.38- and 1.19-fold increases\nin transdermal flux values of carbamazepine when applied as 20% and 30% ethanol solutions across\nmicroneedle-treated porcine skin, respectively, the increases were not statistically significant.
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